
(It honestly depends on a couple of factors for Dest. Why she is staying awake. And what is she doing. The longest so far has been two days. She did minimal physical activity but she was keeping herself busy in other ways. Often the reason she bothers to attempt to stay up for more then a day is when she is having nightmares. Otherwise she doesn’t usually make attempts to avoid sleeping.)

(A day. Lance knows the body needs sleep to function at it’s best. So while he might sleep lighter while on duty, he will sleep. An I don’t mean sleep on duty while at Skyfire. I mean if he is going to be out on a mission where they won’t be back for a day or better. Where sleep is taken in shifts usually.)
(Thanks! @thelittlemonkthatcould )